Hb Estimation - Cyan-MethHb method

Principle 

• Blood is diluted in a solution called Drabkin’s fluid containing potassium ferricyanide and potassium cyanide (KCN).
• The oxy, carboxy and metHb are all converted into cyanmet Hb (HiCN) and there is development of pink colour.
• The intensity of pink colour can be measured in a spectrophotometer or photoelectric colorimeter at 540 nm and this is compared with that of a standard cyanmethaemoglobin solution. 

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